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SPEP & Reflex to IFE Serum

CPT/HCPCS 84155, 84165
Order Code CH844
Turnaround Time 48-72 hrs
Test Includes

If abnormal banding is detected, then Immunofixation will be performed at an additional charge (CPT code(s): 86334).

Specimen Requirements
Specimen Source
Transport Container
(Specimen Container)

Transport Tube

Preferred Specimens
2 mL Serum
Minimum Volume (uL)
1.5 mL
Collection Instructions
(Transport Temperature)
Room Temperature
Specimen Stability
Temperature Period
Room temperature 4 days
Refrigerated 7 days
Frozen 28 days
Reject Criteria

Plasma, Gross hemolysis, Grossly lipemic

Test Details
Capillary Zone Electrophoresis and Spectrophotometry
Clinical Significance

Protein Electrophoresis, with Total Protein and Reflex to IFE, Serum – Serum protein electrophoresis (SPE) is an analytical technique that provides separation of serum protein into six fractions: Albumin, Alpha-1, Alpha-2, Beta-1, Beta-2, and Gamma. Interpretation of elevation, decreased, or visual change in different fractions can be used as a diagnostic aid for a variety of different disease states and protein abnormalities, including monoclonal gammopathies (MG).

Reference Ranges

See Laboratory Report

Alternative Names
Electrophoresis, IFE,SPE with Reflex IFE,SPEP