CPT/HCPCS
85730
Order Code
CA004
Turnaround Time
24 hours
Specimen Requirements
Specimen Source
Plasma
Transport Container
(Specimen Container)
(Specimen Container)
Light Blue (Na-Citrate)
Preferred Specimens
Plasma
Minimum Volume (uL)
90% fill required
Collection Instructions
(Transport Temperature)
(Transport Temperature)
Transport at Room Temperature
Specimen Stability
| Temperature | Period |
|---|---|
| Room temperature | 48 hours (unspun) |
| Refrigerated | |
| Frozen | Unacceptable |
Special Instructions
Blood must be filled to the marked line
Test Details
Methodology
The aPTT reagent consisting of silica mixed with synthetic phospholipid is mixed with the patient plasma. The silica provides a negatively-charged particulate surface for the activation of the contact pathway of coagulation. Calcium chloride is then added to the sample/reagent mixture to initiate clot formation. The time to clot formation is measured photo-optically.
Clinical Significance
The APTT explores the coagulation factors XII, XI, IX, VIII, X, V, II and I except the platelets
Reference Ranges
24.1 – 35.1 seconds
