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Coombs Direct

Order Code CH753
Turnaround Time 48-72 hours
Test Includes

DAT- Direct Coombs Test

Specimen Requirements
Specimen Source
Whole blood
Transport Container
(Specimen Container)

EDTA (lavender-top) tube

Preferred Specimens
Whole blood
Minimum Volume (uL)
Not specified
Collection Instructions
(Transport Temperature)
Transport at Room Temperature
Specimen Stability
Temperature Period
Room temperature 24 hours
Refrigerated 72 hours
Frozen Unacceptable
Reject Criteria

Received frozen, SST tube, Gross hemolysis, REd top tube

Test Details
Immune Agglutination
Clinical Significance

The DAT (Direct Coomb’s test) is positive if red cells have been coated, in vivo, with immunoglobulin, complement, or both. A positive result can occur in immune-mediated red cell destruction, autoimmune hemolytic anemia, a transfusion reaction or in patients receiving certain drugs.

Reference Ranges


Alternative Names
Direct Antiglobulin Test