CPT/HCPCS
82085
Order Code
CH208
Turnaround Time
24 hours
Test Includes
Aldolase Serum
Specimen Requirements
Specimen Source
Serum
Transport Container
(Specimen Container)
(Specimen Container)
SST (Tiger Top)
Preferred Specimens
Serum
Collection Instructions
(Transport Temperature)
(Transport Temperature)
Separate serum or plasma immediately after coagulation (30 minutes). Note: Not removing refrigerated specimens from the clot results in aldolase levels 12% to 46% higher. Transport refrigerated.
Specimen Stability
| Temperature | Period |
|---|---|
| Room temperature | 1 day |
| Refrigerated | 5 days |
| Frozen | 30 days |
Reject Criteria
Hemolysis (red cells contain aldolase)
Test Details
Methodology
Spectrophotometry
Clinical Significance
Aldolase is necessary for glycolysis in muscle as a “rapid response” pathway for production of adenosine triphosphate, independent of tissue oxygen. Aldolase catalyses the conversion of fructose 1,6-diphosphate into dihydroxyacetone phosphate and glyceraldehyde 3- phosphate, an important reaction in the glycolytic breakdown of glucose to lactate in muscle. Aldolase is a tetramer whose primary structure depends upon the tissue from which it was synthesized (liver, muscle, brain). The brain form of aldolase has, because of its preponderance in white cells, been suggested to be a leukemia marker, but this is not confirmed.
Reference Ranges
1.2-7.6 U/L
